Coding

Part:BBa_K1499000:Design

Designed by: Jotthe Kannappan   Group: iGEM14_StanfordBrownSpelman   (2014-08-27)

wssF gene from wss operon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 104
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

This gene was isolated from purified P. fluorescens genomic DNA using the following primers: 

wssF Forward: 5'-TTGTTCGAAGACCTGAGCGG-3'
wssF Reverse: 5'-TTGGCAAAACGCAGGAATGC-3'

The BioBrick prefix and suffix were then added using these primers: 

Forward: 5'-GAATTCGCGGCCGCTTCTAGAG TTGTTCGAAGACCTGAGCGG-3'
Reverse: 5'-CTGCAGCGGCCGCTACTAGTA TTGGCAAAACGCAGGAATGC-3'

Source

The wss operon was extracted from P. fluorescens, an organism that produces cellulose acetate as part of a biofilm that allows it to colonize the air-liquid interface [1].

References

[1] Spiers AJ et al. (2013) Cellulose Expression in Pseudomonas fluorescens SBW25 and Other Environmental Pseudomonads in Cellulose - Medical, Pharmaceutical, and Electronic Applications. DOI: [http://cdn.intechopen.com/pdfs-wm/45637.pdf 10.5772/53736]